Pros | Cons | |
---|---|---|
H&E | Staining protocols are fast Uses a light microscope More resistant to bleaching | Everything is stained, not just specific features Training required to interpret staining |
IHC | Target specific markers compared to H&E Uses a light microscope More resistant to bleaching | Prior knowledge of antigen needed Difficult to multiplex Difficult to investigate colocalized proteins |
IF | Target specific markers compared to H&E Easier multiplexing due to more colours Better for investigating colocalized proteins/multiple markers | Prior knowledge of antigen needed Needs fluorescence-enabled microscope Signal can be bleached by repeated or long exposure to light |
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